In humans, the It has been classified as suspected endocrine disruptors by most countries. IEEE Xplore, delivering full text access to the world's highest quality technical literature in engineering and technology. Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. 22. speculated as a potential bacterium to develop a bioremediation process for radioactive heavy . 23. metal contaminants. Deinococcus geothermalis is capable of reducing Fe (III)-nitrilotriacetic acid, U (VI), and Cr (VI). Deinococcus, originally identified as Micrococcus, are coccoid or rod‐shaped nonsporulating bacteria known for their resistance to multiple stresses and their capacity to repair DNA damage with unparalleled efficiency compared to other known bacterial species.Deinococcus radiodurans strain R1 was the first discovered deinobacteria and was isolated in 1956 (Anderson et al. [11] S. Singh et al., "Phytoremediation of 137 Cesium and 90 Strontium From Solutions and Low-Level Nuclear Waste by Vetiveria zizanoides" Ecotox. 24 radiation and to many other agents that damage DNA. Deinococcus radiodurans has been shown to have a great potential to be used in different fields of investigation. Several microorganisms are already known which have the ability to process a specific toxic chemical but which lack the robustness to stand up to other chemicals which might be present in the … Microbes are small organisms, for example Bacteria, that live D. radiodurans has great potential in terms of application to be used in different fields of investigation, especially bioremediation. Deinococcus radiodurans R1 is one of the most resistant bacteria to chronic ionizing radiation ever identified. While it is known that this resistance is due to exceedingly efficient DNA repair, the molecular mechanisms responsible remain poorly understood. Deinococcus radiodurans is an extremophilic bacterium, one of the most radioresistant organisms known. It can be applied towards the treatment of nuclear energy waste. Therefore, Deinococcus radiodurans shows promise in the bioremediation of mixed radioactive wastes. Types of In Situ Bioremediation: Deinococcus radiodurans What is Bioremediation? Therefore, Deinococcus radiodurans shows promise in the bioremediation of mixed radioactive wastes. Deinococcus radiodurans is the most radiation-tolerant organism ever known. This study investigates the efficiency of a recombinant D. radiodurans (DR1-bf+) strain with an ability to form biofilm for uranium remediation. Biotechnological Application of D. radiodurans in Bioremediation ... Deinococcus radiodurans is unparalleled among all known species in its capacity to overcome oxidative stress that affects all cellular macromolecules (62, 108, 120). In recent years, the extremely radio-resistant Gram positive bacterium, Deinococcus radiodurans, has become an organism of choice for engineering a number of strategies for bioremediation of radioactive waste. | IEEE Xplore Deinococcus radiodurans ("strange berry that withstands radiation", formerly called Micrococcus radiodurans) is an extremophilic bacterium, and is the most radioresistant organism known. Chem Biol 13:387–397, … Cells were grown on HLB medium at 45°C for 6 h and subjected to rapid solvent addition (↓). Description and significance. It has gained importance in recent years as a potential candidate for bioremediation of heavy metals, especially the radioactive type. It can survive cold, dehydration, vacuum, and acid, and is therefore known as a polyextremophile and has been listed as the world's toughest bacterium in The Guinness Book Of World Records. Environ. This . Design and Construction of Deinococcus Radiodurans for Biodegradation of Organic Toxins at Radioactive DOE Waste Sites 4-6 The genus, Deinococcus comprises of a large number of radioresistant species, many of which also display dessication, temperature and metal tolerance (D. … Deinococcus radiodurans, meaning "strange berry that withstands radiation", is a non-pathogenic, Gram-positive aerobic bacteria classified as a member of the family Deinococcaceae. A solvent classified into three groups of log P ow value was added to the cell suspension at 5% (v/v) (a, c, e) and 20% (v/v) (b, d, f). The model organisms tested include D. radiodurans, Pseudomonas putida and Shewanella putrefaciens CN32 [9 ]. 69, 306 (2008). Deinococcus radiodurans is the most radiation-resistant organism known and is also capable of reducing Fe(III)-nitrilotriacetic acid coupled to the oxidation of lactate to CO2 and acetate, and uranium and technetium in the presence of humic acids or synthetic electron shuttle agents (Fredrickson et al., (2000)). Deinococcus radiodurans , is the most radiation tolerant organism ever known, it has gained importance in recent years as a potential candidate for bioremediation of heavy metals, especially radioactive ones. We herein report a new bioremediation method using a radiation-resistant bacterium. [10] H. Brim et al., "Engineering Deinococcus radiodurans For Metal Remediation in Radioactive Mixed Waste Environments," Nat. 18, 85 (2000). Deinococcus radiodurans. 21. organism, being the most radioresistant micro-organism ever known, has always been . Engineering Deinococcus radiodurans for metal remediation in radioactive mixed waste environments Hassan Brim 1, Sara C. McFarlan 2, James K. Fredrickson 3, Kenneth W. Minton 1, Min Zhai 1, Lawrence P. Wackett 2, and Michael J. Daly 1* 1Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814. PloS ONE 3:e3878, 2008; Bai et al. R1 biofilm. Deinococcus radiodurans. OSTI.GOV Journal Article: Engineering Deinococcus geothermailis for Bioremediation of High-Temperature Radioactive Waste Environments Tolerance of Deinococcus geothermalis T27 to toxic organic solvents. In recent years, the extremely radio-resistant Gram positive bacterium, Deinoco-ccus radiodurans, has become an organism of choice for engineering a number of strategies for bioremediation of radioactive waste.4-6 The genus, Deinococcus comprises of a large number of radioresistant species, Bioremediation is the strategy of using bacteria to feed on or simply degrade dangerous compounds. The high cost of remediating radioactive waste sites from nuclear weapons production has stimulated the development of bioremediation strategies using Deinococcus radiodurans, the most radiation resistant organism known. However, the lack of biofilm forming capability proved to be a bottleneck . We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated Abstract. This study investigates the efficiency of a recombinant D. radiodurans (DR1-bf + … Due to its high production and application figures, DBP is commonly found in wastewater, sewage sludge, and aquatic environments. Since D. radiodurans is very resistant to radiation, scientists are interested in using the bacteria to clean up waste sites containing hazardous materials. We have constructed a Deinococcus radiodurans strain harboring phoN , a gene encoding a nonspecific acid phosphatase, obtained from a local isolate of Salmonella enterica serovar Typhi. [1] Not only has D.radiodurans been genetically modified for bioremediation applications, but also it has been discovered that it could perform a major role in … Genetic engineering of radiation-resistant organisms to recover radionuclides/heavy metals from radioactive wastes is an attractive proposition. "Deinococcus radiodurans beats most of the constraints for survival of life on Mars - radiation, cold, vacuum, dormancy, oxidative damage, and other factors," said Dr. Robert Richmond, a research biologist at NASA's Marshall Space Flight Center. Deinococcus radiodurans R1 is one of the most resistant bacteria to chronic ionizing radiation ever identified. Deinococcus radiodurans is the most radiation-tolerant organism ever known. Deinococcus radiodurans. An engineered strain of Deinococcus radiodurans has been shown to degrade ionic mercury and toluene in radioactive mixed waste environments [7]. The bacterium Deinococcus radiodurans is a champion of extreme radiation resistance that is accounted for by a highly efficient protection against proteome, but not genome, damage. bioremediation in such an environment. Nuclear wastes have been demonstrated to host bacteria containing genes for antimicrobial production (Bagwell et al. Actinides, including chelated Pu(IV), U(VI) and Np(V), inhibited growth at millimolar concentrations, suggesting that actinide toxi- Deinococcus radiodurans bioremediation, where the bacteria are used to process dangerous chemicals in hostile environments, involves genetic engineering of D. radiodurans to create a superbug. Biogenic gold nanomaterial-containing Deinococcus radiodurans R1 showed excellent capability for the removal of radioactive iodine (>99%) in several aqueous solutions. Deinococcus radiodurans and other members of the same genus share extraordinary resistance to the lethal and mutagenic effects of ionizing and u.v. Researchers are also trying to modify its function to further consume and … Safe. Biotechnol. A new uranium bioremediation approach using radio-tolerant Deinococcus radiodurans biofilm TMANOBALA 1,,S UDHIR KSHUKLA 2,,TS UBBA RAO 2* and M DHARMENDIRA KUMAR 1 1Department of Applied Science and Technology, Anna University, Chennai 600 025, India 2Biofouling and Thermal Ecology Section, Water and Steam Chemistry Division, BARC Facilities, Kalpakkam 603 102, India We have developed a radiation resistant bacterium for the treatment of mixed radioactive wastes containing ionic mercury. Deinococcus radiodurans is a radiation-resistant extremophile bacterium that is genetically engineered for the bioremediation of solvents and heavy metals. Bioremediation The Environmental Protection Agency (EPA) defines Bioremediation as the use of microbes to clean up soil and ground water. Di-n-butyl phthalate (DBP) is a group of phthalate esters (PAEs) that are widely used in cosmetics, perfumes, and plasticizers. bioremediation programmes addressed the toxicity of actinides, metals and chelators. 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